Genotypic analysis of Bacillus thuringiensis serovars by RAPD-PCR
نویسندگان
چکیده
منابع مشابه
DNA relatedness among Bacillus thuringiensis serovars.
The genetic relationships of Bacillus cereus and of the Bacillus thuringiensis serovars were assessed from measurements of DNA reassociation. A study of 8 to 10 strains each of 13 of the most commonly encountered serovars revealed that the levels of intragroup DNA relatedness for most serovars ranged from 90 to 100%. In contrast, B. thuringiensis serovars canadensis and kenyae consisted of two ...
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Many strains of Bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen B. thuringiensis has also been involved in outbreaks of diarrhea. The diarrheal types of diseases are attributed to enterotoxins. Two different enterotoxic protein complexes, hemolysin BL (HBL) and nonhemolytic enterotoxin (NHE), and an enterotoxic protein, enterotoxin T, have been character...
متن کاملParentage analysis using RAPD PCR.
DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...
متن کاملMolecular characterization of Bacillus thuringiensis using rep-PCR
The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups were formed when 45% was the average distance of the population: group 1 with 41,5% of the isolate...
متن کاملPCR Detection of cry Genes in Local Bacillus Thuringiensis Isolates
Twelve local B. thuringiensis strains are used in the study, which previously isolated from the soil of seven governorates and characterized by their high toxicity against 2 instars larvae of cotton nd leafworm (Spodoptera littoralis). The PCR amplification analysis of the 12 Bt isolates revealed the presence of amplified fragments characteristic of five different cry genes. Results showed the ...
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ژورنال
عنوان ژورنال: Neotropical Biology and Conservation
سال: 2010
ISSN: 1809-9939
DOI: 10.4013/nbc.2010.52.06